nexterion® h microarray slides  (SCIENION)

Journal: PLOS ONE

Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

doi: 10.1371/journal.pone.0283537

Figure Lengend Snippet: Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) microarray, the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.

Article Snippet: Nexterion® slide H microarray slides were supplied by Schott AG (Mainz, Germany).

Techniques: Recombinant, Construct, Microarray, Expressing, Sequencing, Concentration Assay, Binding Assay, Membrane

Journal: PLOS ONE

Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

doi: 10.1371/journal.pone.0283537

Figure Lengend Snippet: (A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).

Article Snippet: Nexterion® slide H microarray slides were supplied by Schott AG (Mainz, Germany).

Techniques: Microarray, Incubation, Purification, Binding Assay, SDS Page, Staining

Journal: PLOS ONE

Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

doi: 10.1371/journal.pone.0283537

Figure Lengend Snippet: Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) microarray, the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.

Article Snippet: All protein antigens in PBS, pH 7.4 ( ) were printed on Nexterion® H microarray slides in six replicate features, approximately 1 nL per feature (2 drops), with 8 replicate subarrays per microarray slide using a Scienion SciFlexArrayer S3 essentially as previously described [ ].

Techniques: Recombinant, Construct, Microarray, Expressing, Sequencing, Concentration Assay, Binding Assay, Membrane

Journal: PLOS ONE

Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

doi: 10.1371/journal.pone.0283537

Figure Lengend Snippet: (A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).

Article Snippet: All protein antigens in PBS, pH 7.4 ( ) were printed on Nexterion® H microarray slides in six replicate features, approximately 1 nL per feature (2 drops), with 8 replicate subarrays per microarray slide using a Scienion SciFlexArrayer S3 essentially as previously described [ ].

Techniques: Microarray, Incubation, Purification, Binding Assay, SDS Page, Staining